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Becton Dickinson
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Bio-Rad
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Jackson Immuno
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Proteintech
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Image Search Results
Journal: Cancer Research
Article Title: A Novel Gene Expression Profile in Lymphatics Associated with Tumor Growth and Nodal Metastasis
doi: 10.1158/0008-5472.can-07-6506
Figure Lengend Snippet: Figure 2. Confirmation of altered gene expression in lymphatics of T241/VEGF-C fibrosarcomas by comparative immunostaining. A, representative frozen sections of normal dermis and primary tumors from mice transplanted with either T-241 control or T-241/VEGF-C fibrosarcomas were dual immunostained for LYVE-1 (Alexa 488; green) and ESAM, Endoglin, or CD200 (Alexa 568; red) to confirm the up-regulated gene expression seen in Affymetrix microarray analyses of isolated LEC (Fig. 1C). ESAM, Endoglin, and CD200 expression is restricted to LYVE-1–negative blood capillaries in normal skin and nonmetastatic control T-241 tumors but selectively up-regulated in intratumoral/peritumoral LYVE-1–positive lymphatic capillaries in metastatic T-241/VEGF-C fibrosarcomas (original magnification, 20). B, dual immunostaining for LYVE-1 and biglycan shows that the proteoglycan is abundant in the subendothelial matrix of LYVE-1–negative blood vessels and small LYVE-1–positive lymphatic capillaries in normal dermis and control nonmetastatic T-241 tumors but is absent from LYVE-1–positive lymphatics in metastatic T241/ VEGF-C fibrosarcomas (original magnification, 20). Arrowheads in selected images are included as points of reference for appropriate single or dual stained lymphatic capillaries.
Article Snippet: Polyclonal goat anti-human ESAM, goat antihuman LYVE-1, and goat anti-mouse VEGF receptor 3 (VEGFR3) antibodies were from R&D Systems Europe; mouse anti-human Endoglin was from DAKO; polyclonal rabbit anti-human leptin-R antibodies were from Abcam; rat anti-mouse CD31, Endoglin, and MECA-32 monoclonal antibodies were from BD Pharmingen; rabbit anti-Prox1 was from Research Diagnostics Inc.; goat anti-mouse biglycan was from Santa Cruz Biotechnology; and
Techniques: Gene Expression, Immunostaining, Control, Microarray, Isolation, Expressing, Staining